Improved immobilization of DNA to microwell plates for DNA-DNA hybridization.
نویسندگان
چکیده
An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional method. The overall hybridization efficiency was increased 3.1-5.2 times. This simple new protocol can reduce the consumption of scarce DNA samples.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 24 20 شماره
صفحات -
تاریخ انتشار 1996